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p38 ktr mef2c mcerulean3 covert lab6 addgene  (Addgene inc)


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    Addgene inc p38 ktr mef2c mcerulean3 covert lab6 addgene
    P38 Ktr Mef2c Mcerulean3 Covert Lab6 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38 ktr mef2c mcerulean3 covert lab6 addgene/product/Addgene inc
    Average 93 stars, based on 9 article reviews
    p38 ktr mef2c mcerulean3 covert lab6 addgene - by Bioz Stars, 2026-02
    93/100 stars

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    Figure 1. Optimization and Application of the Estrogen Receptor 𝛼𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛼𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛼𝛼FL CyOFP1-EV biosensor (EV; n = 39) and the ER 𝛼𝛼FL <t>CyOFP1-P2A</t> biosensor (P2A; n = 21) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001). Mean values are shown above each bar. D) Time course of mean normalized FRET/ECFP emission ratio changes and E) representative images illustrate the FRET ratios of the ER 𝛼𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 26) and the ER 𝛼𝛼FL FRET biosensor (mNeonGreen; n = 27) before and after treatment
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    Figure 1. Optimization and Application of the Estrogen Receptor 𝛼𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛼𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛼𝛼FL CyOFP1-EV biosensor (EV; n = 39) and the ER 𝛼𝛼FL <t>CyOFP1-P2A</t> biosensor (P2A; n = 21) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001). Mean values are shown above each bar. D) Time course of mean normalized FRET/ECFP emission ratio changes and E) representative images illustrate the FRET ratios of the ER 𝛼𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 26) and the ER 𝛼𝛼FL FRET biosensor (mNeonGreen; n = 27) before and after treatment
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    Figure 1. Optimization and Application of the Estrogen Receptor 𝛼𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛼𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛼𝛼FL CyOFP1-EV biosensor (EV; n = 39) and the ER 𝛼𝛼FL <t>CyOFP1-P2A</t> biosensor (P2A; n = 21) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001). Mean values are shown above each bar. D) Time course of mean normalized FRET/ECFP emission ratio changes and E) representative images illustrate the FRET ratios of the ER 𝛼𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 26) and the ER 𝛼𝛼FL FRET biosensor (mNeonGreen; n = 27) before and after treatment
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    Figure 1. Optimization and Application of the Estrogen Receptor 𝛼𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛼𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛼𝛼FL CyOFP1-EV biosensor (EV; n = 39) and the ER 𝛼𝛼FL CyOFP1-P2A biosensor (P2A; n = 21) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001). Mean values are shown above each bar. D) Time course of mean normalized FRET/ECFP emission ratio changes and E) representative images illustrate the FRET ratios of the ER 𝛼𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 26) and the ER 𝛼𝛼FL FRET biosensor (mNeonGreen; n = 27) before and after treatment

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Novel FRET-Based Biosensors for Real-Time Monitoring of Estrogen Receptor Dimerization and Translocation Dynamics in Living Cells.

    doi: 10.1002/advs.202406907

    Figure Lengend Snippet: Figure 1. Optimization and Application of the Estrogen Receptor 𝛼𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛼𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛼𝛼FL CyOFP1-EV biosensor (EV; n = 39) and the ER 𝛼𝛼FL CyOFP1-P2A biosensor (P2A; n = 21) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001). Mean values are shown above each bar. D) Time course of mean normalized FRET/ECFP emission ratio changes and E) representative images illustrate the FRET ratios of the ER 𝛼𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 26) and the ER 𝛼𝛼FL FRET biosensor (mNeonGreen; n = 27) before and after treatment

    Article Snippet: Next, the EV linker and P2A peptide sequences were amplified from pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5 (Addgene plasmid #111818) and pCAGGS-6011nes (Addgene plasmid #108652) before being digested with BspEI/MluI.

    Techniques:

    Figure 2. Optimization and Application of the Estrogen Receptor 𝛽𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛽𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛽𝛼FL CyOFP1-EV biosensor (EV; n = 33) and the ER 𝛽𝛼FL CyOFP1-P2A biosensor (P2A; n = 33) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001, ns: not significant). D) Time course of changes in the mean normalized FRET/ECFP emission ratio and E) representative images illustrating the FRET ratios of the ER 𝛽𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 49) and the ER 𝛽𝛼FL FRET biosensor (mNeonGreen; n = 14) before and after treatment with 10

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Novel FRET-Based Biosensors for Real-Time Monitoring of Estrogen Receptor Dimerization and Translocation Dynamics in Living Cells.

    doi: 10.1002/advs.202406907

    Figure Lengend Snippet: Figure 2. Optimization and Application of the Estrogen Receptor 𝛽𝛼Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛽𝛼FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio of the ER 𝛽𝛼FL CyOFP1-EV biosensor (EV; n = 33) and the ER 𝛽𝛼FL CyOFP1-P2A biosensor (P2A; n = 33) after 30 min of treatment with 10 μM 4-hydroxytamoxifen (****p < 0.0001, ns: not significant). D) Time course of changes in the mean normalized FRET/ECFP emission ratio and E) representative images illustrating the FRET ratios of the ER 𝛽𝛼FL CyOFP1-P2A biosensor (CyOFP1; n = 49) and the ER 𝛽𝛼FL FRET biosensor (mNeonGreen; n = 14) before and after treatment with 10

    Article Snippet: Next, the EV linker and P2A peptide sequences were amplified from pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5 (Addgene plasmid #111818) and pCAGGS-6011nes (Addgene plasmid #108652) before being digested with BspEI/MluI.

    Techniques:

    Figure 3. Optimization and Application of the Estrogen Receptor 𝛽𝛽Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛽𝛽FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio between the ER 𝛽𝛽FL CyOFP1-EV (EV; n = 31) and the ER 𝛽𝛽FL CyOFP1-P2A biosensors (P2A; n = 21) after treatment with 10 μM 17𝛽-estradiol for 30 min (****p < 0.0001, *p < 0.05). D) Time course of changes in the mean normalized FRET/ECFP emission ratio and E) representative images illustrating the FRET ratios of the ER 𝛽𝛽 FL CyOFP1-EV (CyOFP1; n = 31) and ER 𝛽𝛽FL FRET biosensors (mNeonGreen; n = 17) before and after treatment with 10 μM 17𝛽-estradiol (****p

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Novel FRET-Based Biosensors for Real-Time Monitoring of Estrogen Receptor Dimerization and Translocation Dynamics in Living Cells.

    doi: 10.1002/advs.202406907

    Figure Lengend Snippet: Figure 3. Optimization and Application of the Estrogen Receptor 𝛽𝛽Full-Length (FL) FRET Biosensor. A) Schematic representation and B) mode of action of ER 𝛽𝛽FL FRET biosensors. C) Bar graphs depict changes in the normalized FRET/ECFP emission ratio between the ER 𝛽𝛽FL CyOFP1-EV (EV; n = 31) and the ER 𝛽𝛽FL CyOFP1-P2A biosensors (P2A; n = 21) after treatment with 10 μM 17𝛽-estradiol for 30 min (****p < 0.0001, *p < 0.05). D) Time course of changes in the mean normalized FRET/ECFP emission ratio and E) representative images illustrating the FRET ratios of the ER 𝛽𝛽 FL CyOFP1-EV (CyOFP1; n = 31) and ER 𝛽𝛽FL FRET biosensors (mNeonGreen; n = 17) before and after treatment with 10 μM 17𝛽-estradiol (****p

    Article Snippet: Next, the EV linker and P2A peptide sequences were amplified from pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5 (Addgene plasmid #111818) and pCAGGS-6011nes (Addgene plasmid #108652) before being digested with BspEI/MluI.

    Techniques:

    Figure 4. Optimization and Application of the Estrogen Receptor 𝛽𝛽LBD FRET Biosensor. A) Schematic diagram and B) mode of action of ER 𝛽𝛽LBD FRET biosensors. C) Bar graphs depict changes in the normalized FRET/mTurquoise2 emission ratio between the ER 𝛽𝛽LBD N-N term-EV (EV; n = 5) and the ER 𝛽𝛽LBD N-N term-P2A biosensors (P2A; n = 5) after treatment with 10 μM 17𝛽-estradiol for 30 min (****p < 0.0001, **p < 0.01). D) Time course of changes in the mean normalized FRET/mTurquoiuse2 emission ratio and E) representative images illustrating the FRET ratios of the ER 𝛽𝛽 LBD N-N term-EV (N-N; n = 5) and the ER 𝛽𝛽LBD FRET biosensors (N-C; n = 13) before and after treatment with 10 μM 17𝛽-estradiol (****p < 0.0001),

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Novel FRET-Based Biosensors for Real-Time Monitoring of Estrogen Receptor Dimerization and Translocation Dynamics in Living Cells.

    doi: 10.1002/advs.202406907

    Figure Lengend Snippet: Figure 4. Optimization and Application of the Estrogen Receptor 𝛽𝛽LBD FRET Biosensor. A) Schematic diagram and B) mode of action of ER 𝛽𝛽LBD FRET biosensors. C) Bar graphs depict changes in the normalized FRET/mTurquoise2 emission ratio between the ER 𝛽𝛽LBD N-N term-EV (EV; n = 5) and the ER 𝛽𝛽LBD N-N term-P2A biosensors (P2A; n = 5) after treatment with 10 μM 17𝛽-estradiol for 30 min (****p < 0.0001, **p < 0.01). D) Time course of changes in the mean normalized FRET/mTurquoiuse2 emission ratio and E) representative images illustrating the FRET ratios of the ER 𝛽𝛽 LBD N-N term-EV (N-N; n = 5) and the ER 𝛽𝛽LBD FRET biosensors (N-C; n = 13) before and after treatment with 10 μM 17𝛽-estradiol (****p < 0.0001),

    Article Snippet: Next, the EV linker and P2A peptide sequences were amplified from pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5 (Addgene plasmid #111818) and pCAGGS-6011nes (Addgene plasmid #108652) before being digested with BspEI/MluI.

    Techniques: